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Download Enzymes in Industry: Production and Applications by Wolfgang Aehle PDF

By Wolfgang Aehle

Best specialists from worldwide current an summary of using enzymes in for: the construction of bulk items, akin to glucose, or fructose meals processing and nutrients research laundry and automated dishwashing detergents the cloth, pulp and paper and animal feed industries medical prognosis and remedy genetic engineering. The ebook additionally covers identity equipment of latest enzymes and the optimization of recognized ones, in addition to the regulatory features for his or her use in commercial functions. updated and huge in scope, it is a probability for non-specialists to acquaint themselves with this quickly transforming into box. "...The so nice that there's no hesitation in recommending it as perfect examining for any pupil requiring an advent to enzymes.... Enzymes in - may still command a spot in any library, commercial or educational, the place will probably be usually used." —The Genetic Engineer and Biotechnologist

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Extra resources for Enzymes in Industry: Production and Applications

Example text

The increase in absorbance per minute at 500 nm is measured to determine the activity of glycerol phosphate oxidase. 4 Fluorometry [78] The fluorometric method is rarely used for determining the catalytic activity of raw or purified enzyme preparations. Because of its high sensitivity, it permits the assay of small amounts of enzymes in organs or tissue sections [79]. For example, systems that depend on NAD and NADP can be measured by fluorometry; the reduced pyridine coenzymes exhibit a fluorescence of low intensity.

Bottles and stoppers used for lyophilized enzymes must be absolutely tight to prevent access of moisture. Glass or plastic bottles as well as stoppers (rubber or plastic) should not release any traces of heavy metals or other enzyme-inactivating substances into the enzyme solution or suspension. In some cases, enzymes must be protected from light and packaged in brown glass bottles. 1 Microbial Production Use of an aerobic submerged culture in a stirred-tank reactor is the typical industrial process for enzyme production involving a microorganism that produces mostly an extracellular enzyme.

Beyond this, recent results suggest that RNA enzymes are ideally suited for exploration of the energetic origins of this interconnection between binding and catalysis [51]. This use of binding energy provides a natural connection between rate enhancement and specificity, the two hallmarks of biological catalysis. Finally, ribozymes will not only offer new clues about evolution [52], but also offer the potential for specific inactivation of disease-associated mRNAs or viral RNA genomes that, unlike conventional therapeutics, require no knowledge of the structure or function of proteins that target RNAs encode [53].

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