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Download Diagnostic Procedure in Veterinary Bacteriology and Mycology by Grace R. Carter, John R. Cole Jr. PDF

By Grace R. Carter, John R. Cole Jr.

This new version of a typical reference contains classical tools and knowledge on more recent applied sciences, resembling DNA hybridization and monoclonal antibodies.

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Lysis may occur at low dilutions with some serovars. The vaccination history of the herd must be considered in evaluating results. End points of 1:1000 or greater can be detected in animals that have been recently vaccinated (11,12). E. Screening test: If a large number of sera are to be tested, it may be desirable to screen them first at the 1:50 dilution to eliminate the negatives. ). , pomona in row A, hardjo in row B, and so on). This procedure allows 12 sera to be screened against 8 antigens on one plate.

5-3). Rectal swabs or feces may be utilized for examination from a live animal, but numbers of organisms in the feces may be small and the spirochetes not readily detectable (15). Isolation Procedures Treponema hyodysenteriae can be isolated from the intestinal mucosa using the following procedure (16,17). 5. Spirochetes 53 Figure 5-3. Treponema hyodysenteriae in a section of swine intestine. Warthin-Starry stain, x - 1 0 0 0 . 1. Take 6-8 in of spiral colon from an acutely affected animal. The sample may be held for 2-3 days at 4°C before isolation attempts.

Washington, J. , Balows, A. and Sonnewirth, A. C : Collection, handling and processing of specimens. In Lennette, Ε. H. -in-chief): Manual of Clini­ cal Microbiology, 4th ed. Washington, D. C , American Society for Microbiology, 1985. Some do not grow on MacConkey agar; exceptions listed. O-F medium; fermentation observable in triple sugar iron medium. ^Cytochrome (indophenol) oxidase test. Lactose is valuable in the case of prompt fermenters (on differential plates overnight, or in 24-48 hr in fermentation media); some strains of the groups listed show delayed or no fermentation of lactose.

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