Posted in Cancer

Download Cancer and Zebrafish: Mechanisms, Techniques, and Models by David M. Langenau PDF

By David M. Langenau

This quantity makes a speciality of defining the original attributes of utilizing the zebrafish melanoma version for locating vital pathways and power drug pursuits for the remedy of human cancers. utilizing the zebrafish version, the quantity explores oncogene and tumor suppressor discovery, chemical genetic techniques, genomics, epigenetics, melanoma imaging, and cellphone transplantation. Contributed chapters come from the main trendy laboratories operating during this box, which supplies a distinct viewpoint on zebrafish versions from a large spectrum of the study group. additionally, the publication bargains a close research of the most up-tp-date learn within the zone for particular zebrafish melanoma versions, together with T cellphone leukemia, rhabdomyosarcoma, liver and pancreatic melanoma, cancer, neuroblastoma, germ mobilephone tumors, and malignant peripheral sheath tumors. A bankruptcy can also be devoted to the improvement and usage of alternative piscine versions of melanoma. The compilation of chapters within the quantity culminates right into a finished and definitive textual content on zebrafish and melanoma, offering a miles wanted source at the strong attributes of the zebrafish version system.

Show description

Read Online or Download Cancer and Zebrafish: Mechanisms, Techniques, and Models PDF

Similar cancer books

DNA Topoisomerases in Cancer Therapy: Present and Future

Within the mid 80's variety I and II enzymes have been came across to be the intracellular objectives of a couple of efficacious anticancer medicinal drugs akin to doxorubicin, mitoxantrone, etoposide and camptothecin because of a endured efforts of many investigators, specifically Leroy Liu and his collaborators at Johns Hopkins college.

Cancer Chemoprevention: Promising Cancer Chemopreventive Agents

A lot development has been made in researching and constructing brokers that experience promise, or have already been effectively used, to regard precancerous stipulations or inhibit carcinogenesis. In melanoma Chemoprevention, quantity 1: Promising melanoma Chemopreventive brokers, top researchers within the discovery and improvement of chemopreventives comprehensively survey all facets of those rising therapeutics.

Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy

Taken jointly the information awarded during this overview, and paintings via many different investigators, aid the thought that DNA excision fix is critical in a tumor cell's resistance to platinum compounds. Inhibition of this fix method by way of blend chemotherapy with the excision fix inhibitors HU and Ara-C produces synergistic mobilephone kills and elevated degrees and persistance of DNA interstrand crosslinks.

Cancer and Zebrafish: Mechanisms, Techniques, and Models

This quantity makes a speciality of defining the original attributes of utilizing the zebrafish melanoma version for locating very important pathways and capability drug objectives for the remedy of human cancers. utilizing the zebrafish version, the quantity explores oncogene and tumor suppressor discovery, chemical genetic techniques, genomics, epigenetics, melanoma imaging, and cellphone transplantation.

Additional resources for Cancer and Zebrafish: Mechanisms, Techniques, and Models

Sample text

G) Increased transgene expression is achieved by application of up to 5 UAS repeats, while a much higher copy number reduces transcription efficiency. Systems similar to Gal4/UAS include the Q-system (H) and the TrpR/tUAS system (I) composed of the transactivator (pink oval) QF/TrpR and the upstream activation sequence (yellow rectangle) QUAS/tUAS, respectively. The QF can be inhibited by QS (blue half circle). (J) As these systems do not cross-react they can be combined in one model to study cell non-autonomous effects for example by expressing Gal4 and QF under different cell type specific promoters to elucidate the effect of disabled Notch-signaling (through expression of dominant-negative suppressor of hairless, grey rectangle) in B-cells, and assess the effect on oncogenic behavior of T-cells.

Proposed a method for RMCE in zebrafish through phiC31 [95]. Using a tol2 construct they expressed attP flanked GFP under a tissue specific promoter and could exchange it with an attB flanked mCherry. A drawback however is the relatively low efficiency of phiC31 integrase in genomic approaches. Another approach described by Mosimann et al. aims to establish single-insertion zebrafish lines that harbor only one attP site [96]. 34 M. Mayrhofer and M. Mione They developed three functional lines with single attP integration sites of which they characterized two with homogenous gene expression.

Through injection of two different gRNAs and Cas9 mRNA even huge chromosomal deletions are possible [120]. To reduce mutation events the knock-out efficiency can also be titrated through varying RNA concentrations [118]. As the mutations are inherited to the next generation the CRISPR/Cas9 system allows to generate stable knock-out lines with a fast and easy protocol [121]. While off-target effects have been found to appear in only few cases, still deeper investigation is required [119]. However, the system displays variable efficiencies for different genomic targets with some genes yielding effects in <1 % of injected embryos [117, 118, 122].

Download PDF sample

Rated 4.16 of 5 – based on 34 votes