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Presence of ammonium was primarily tested using Nesler’s reagent before the concentration was determined by ion chromatography. Table 1. OD, pH and ammonium accumulation by A. beijerinckii and A. vinelandii in GNFMM liquid medium of various glucose concentrations after 2 days incubation. A. 041 A. 0)* N. D. 0)* N. D. 2)* N. D. 2)* N. D. : not determined, OD: optical density (600 nm). *Figures in parentheses show the value before incubation. Note: ammonium ion concentration is in mM. Presence of ammonium was primarily tested using Nesler’s reagent before the concentration was determined by ion chromatography.

Woodley, P. & Drummond, M. (1999). The basis of ammonium release in nifL mutants of Azotobacter vinelandii. , Botvinko, I. V. & Egorov, N. S. (1992). Production of extracellular polysaccharides by Azotobacter beijerinckii. , Yamakawa, H. & Omori, T. (2010). Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lisobacter sp. E4. Journal of Bioscience and Bioengineering 110 (4), 415-418 24 Biotechnology - Molecular Studies and Novel Applications for Improved Quality of Human Life Kennedy, I.

From the economic point of view the marine algae represent an important resource of food and industrial input. The Caribbean Sea coast of Colombia contains innumerable species that have an economic value and are used as human food, medicinal products, fertilizers, fuel, and play an important role in the extraction of phycocolloids and hydrocolloids (Teas, 2007). All these products have a big industrial application. In spite of the speculation on the seaweed potential as a direct source of proteins and pharmaceutical products, the demand for phycocolloids will be the factor that will influence the future development of the marine algae world resources.

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