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By Milton Zaitlin

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3S) was sedimented in a separate tube during the same run. Somatic embryo proteins of celery were extracted, sedimented, and fractionated using the same procedures and compared to the 8S and 12S celery seed proteins. Celery somatic embryo proteins do not accumulate in distinct peaks as do celery seed proteins. As a result, regions corresponding to the 8S and 12S portions of the gradient were pooled for SDS-PAGE analysis (Fig. 7). Celery seed protein can be seen in the total protein and in the 8S and 12S fractions.

4). 5 mM ammonium. We generally supply 5 mM to avoid the preciptious inhibition apparent at higher levels of NH^ . It should be noted that many of the standard plant tissue culture media supply higher or lower amounts of NH, than is optimal for celery regen­ eration (Gamborg ejt a_l. , 1976;. Somatic Embryos of Alfalfa and Celery 41 AMMONIUM (mM) Fig. 4. The response of embryogenic celery callus cells to the level of NH^ in the regeneration medium. 5 μΜ kinetin for 21 days. 87o agar solidified medium containing various levels of (NH^)^ S0^.

A) Transverse section of anther at start of culture, showing uninucleate microspores arranged in peripheral distribution around interior of loculus with pore adpressed to tapetal surf­ ace. One irregular microspore, spherical in shape, is shown in interior of loculus and not in contact with tapetum. (b) Trans­ verse section of anther after period in culture. Most spores in the peripheral position have shrunk or collapsed in shape. Two have undergone embryogenic divisions and enlarged. Different types of partitioning are shown in these 2 structures.

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