By Sang Yup Lee, Jin Hwan Park (auth.), Christoph Wittmann, Rainer Krull (eds.)
-Integration of platforms Biology with Bioprocess Engineering: L-Threonine construction by means of structures Metabolic Engineering of Escherichia Coli, by means of Sang Yup Lee and Jin Hwan Park; -Analysis and Engineering of Metabolic Pathway Fluxes in Corynebacterium glutamicum, by means of Christoph Wittmann; -Systems Biology of commercial Microorganisms, Marta Papini, Margarita Salazar, and Jens Nielsen; -De Novo Metabolic Engineering and the Promise of artificial DNA, via Daniel Klein-Marcuschamer, Vikramaditya G. Yadav, Adel Ghaderi, and Gregory N. Stephanopoulos; -Systems Biology of Recombinant Protein creation in Bacillus megaterium, Rebekka Biedendieck, Boyke Bunk, Tobias Fürich, Ezequiel Franco-Lara, Martina Jahn, and Dieter Jahn; -Extending man made Routes for Oligosaccharides through Enzyme, Substrate and response Engineering; by way of Jürgen Seibel, Hans-Joachim Jördening, and Klaus Buchholz; -Regeneration of Nicotinamide Coenzymes: rules and functions for the Synthesis of Chiral Compounds; by means of Andrea Weckbecker, Harald Gröger, and Werner Hummel;
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Additional resources for Biosystems Engineering I: Creating Superior Biocatalysts
Metabolism of Corynebacterium glutamicum . . . . . . . . . . . . . . . . . . . . . . . 1 Nutritional Requirements and Assimilatory Pathways . . . . . . . . . . . . . . . . 2 Central Carbon Metabolism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 23 23 23 C. de 22 C. 3 Biosynthetic Pathways Towards Biotechnology Products . . . . . . . . . . . . . . 4 Genome Sequencing and Metabolic Network Reconstruction .
2). An important branch point occurs at the level of aspartate semialdehyde, where the biosynthetic pathways separate. Upstream of this node is a key point for flux control of lysine biosynthesis. The responsible enzyme is aspartokinase, which catalyzes the formation of aspartylphosphate from aspartate. It is subjected to feedback inhibition by lysine and threonine [36, 37]. Downstream of this node, C. glutamicum exhibits a dual pathway for lysine biosynthesis, providing an increased flexibility in response to changing environmental conditions [38, 39].
The availability of genome information enabled new post genome technologies accelerating strain engineering by systems biology approaches. These comprise experimental and computational tools such as transcriptomics [48–51], proteomics [52–54], or in < Fig. 2 Metabolic pathways in C. glutamicum for biosynthesis of the aromatic amino acids tryptophan, tyrosine, and phenylalanine (a) and amino acids belonging to the aspartate family including lysine, methionine, threonine, and isoleucine (b). Metabolic regulation by feedback inhibition is indicated by dotted lines 28 C.