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By Ashok Pandey, Jo-Shu Chang, Patrick C Hallenbeck and Christian Larroche (Eds.)

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USA 108 (10), 3941–3946. , 2011. Photobiological hydrogen production: Recent advances and state of the art. Bioresour. Technol. 102 (18), 8403–8413. , 2011. Efficient H-2 production via Chlamydomonas reinhardtii. Trends Biotechnol. 29 (12), 595–600. , 2010. Electricity-mediated biological hydrogen production. Curr. Opin. Microbiol. 13 (3), 307–315. , 2009. Fermentative hydrogen yields from different sugars by batch cultures of metabolically engineered Escherichia coli DJT135. Int. J. Hydrogen Energy 34 (19), 7979–7982.

106, 154–160. , 2012c. Near stoichiometric reforming of biodiesel derived crude glycerol to hydrogen by photofermentation. Int. J. Hydrogen Energy 37 (3), 2273–2277. , 2011. Multiple facets of anoxic metabolism and hydrogen production in the unicellular green alga Chlamydomonas reinhardtii. New Phytol. 190 (2), 279–288. , 2009. Fermentative hydrogen production: Principles, progress, and prognosis. Int. J. Hydrogen Energy 34 (17), 7379–7389. , 2011. Microbial paths to renewable hydrogen production.

This simple and powerful method has been called recombineering and allows insertional inactivation without intermediate cloning steps. This system has been well developed for E. , 2009) and allows the systematic analysis of unknown gene function and gene regulatory networks, as well as genome-wide testing of mutational effects in a common background. Many different mutational studies have been carried out with E. , 2011, 2012). There is a great deal of interest in the Gram-positive Firmicutes, well known for their powers of fermentation, but until relatively recently most have been difficult to work with genetically so very few studies have been reported on the metabolic engineering of biohydrogen production.

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