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Download Air Force Colors 1926 - 1942 Vol. I by Dana Bell PDF

By Dana Bell

Ninety six pages, learn folks army plane colour schemes from 1926 to 1942, bw and colour illustrations

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Coli strains with amber suppressor tRNA, such as XL1Blue. (c) Downstream sequence of pAK300scFv, pAK400scFv, pJB23scFv, and pJB33scFv. The last two bases of VH (bold), the SfiI and EcoRI restriction sites and (His)6 tag (boxed) are indicated above the sequence. (d) Sequences of the downstream EcoRI/HindIII fusion cassettes as used in pAK500 and pAK600. The dHLX dimerization motif (double underlined) was taken from Pack et al. (1993). The complete sequence of the mature E. coli alkaline phosphatase (AP) gene can be found in Shuttleworth et al.

3. Digest appropriate amounts of vector (pAK100 or pJB12, see Fig. 4) with SfiI in the presence of the above-mentioned buffer, including BSA. Use 10 units SfiI for 1 mg vector in 50 ml and incubate 4 h at 50 C. 5 unit/mg vector) to the digestion mix after 2 h and continue incubation for another 2 h at 50 C. Note: Dephosphorylation should not be necessary because of the asymmetric overhangs. However, we always include this step to eliminate any risk of religation of single-cut vector. Note: pAK100 or pJB12 are phage display vectors (Fig.

PAK400scFv, pJB33scFv ® SD2 pelB signal sequence . . GA AGGAGA T A T A C A T A T GA A A T A CC T A T T GCC T A CGGC AGCC . . M T7g10 K Y L L P T A A . . b pAK100scFv ¬ VH EcoRI Sfi I myc tag . . CGGC C T CGGGGGC CG A A T T C G A GC A G A A GC T G A T C T C T G A GG A A G A C . . A S G ® gene III 250-406 A E F E Q K L I S E E D C T G T AGGG T GG T GGC T C T GG T T CCGG T G A T T T T GA T T A T GA A A AG . . L * G G G S G S G D F D Y E K . . pJB12scFv ¬ VH EcoRI Sfi I trypsin cleavage site .

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